Abstract
Objective:
Acute lung injury is a complication after hepatic ischemia/reperfusion (I/R) and might be responsible for high mortality rate. The aim of this study was to demonstrate that adrenomedulline administration may prevent lung injury after hepatic I / R by downregulation of proinflammatory cytokines.
Materials and Methods:
This study was performed by using 54 male Wistar rats. The rats were randomly allocated into 3 groups and in groups were randomly allocated into 1st, 2nd, and 4th hour subgroups. After I/R, AM (12μg/kg) was infused for 30 minute via portal vein. Blood and tissue samples were collected 1, 2 and 4 hour after reperfusion. Hepatic I/R induced lung injury, as characterized by lung edema, histopathologic changes and proinflammatory cytokines including tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels were evaluated.
Results:
The TNF-α enzyme activity in the first hour was found to be significantly higher in the I/R group than in the Sham and AM group (p=0.027 and p=0.041, respectively). The levels of TNF-α in the sham-operated group were similar in the AM group (p=0.310). Significant differences were found only in the second hour IL-6 measurements (p=0.038). Similar differences in caspase-9 enzyme activity in the sham-operated and AM group (p=0.291). Treatment with AM decreased lung injury after hepatic I/R as demonstrated by reduction in histologic damage.
Conclusions:
Acute lung injury was decreased by AM treatment after hepatic I/R injury.
Keywords: Adrenomedullin, hepatic ischemia/reperfusion, lung injury
