ABSTRACT

The genetic polymorphism found in the Ukrainian population is the basis for the recommendation of the genetic testing of 163A/C polymorphism of the CYP1A2 gene in the prescribing drugs - substrates of this gene.

The following distribution of genotypes in a sampling of the Ukrainian population under the 163A/C polymorphism of the CYP1A2 gene: AA - 36%, AC - 49% and CC - 15% in the subjects was revealed. The population allele frequencies of the 163A/C polymorphism of the CYP1A2 gene totaled pА = 0.6 and qС = 0.4. The population structure of the individuals does not deviate from the Hardy-Weinberg equilibrium, as may be seen from the lack of difference between the theoretically expected and the observed frequencies of the three genotypes.

Total sampling of genetic material (buccal epithelium) was collected in 102 subjects (48 males, 54 females) not related to each other. Genotype test of the participants of the study for CYP1A2 polymorphism (rs762551) was performed using the polymerase chain reaction. In accordance with the findings of genotyping the allele frequencies (р and q) were calculated.

CYP1A2 is a very important gene for a potential genotyping. The objective of this work was to study the population frequencies of the corresponding 163A/C single nucleotide polymorphism of the CYP1A2 gene in the sampling of the Ukrainian population.