ABSTRACT

Purpose:

Survivin, an inhibitor of the apoptosis (IAP) gene family, is a mitotic prote-in with actions on various cellular events such as cell division, apoptosis, and cellular response to stress. Vincristine, a chemotherapeutic agent used in multiple myeloma (MM), induces apoptosis via inhibition of spindle formation. We aimed to detect the expression pattern of survivin in vincristine treated MM cell line ARH77.

Materials and Methods:

Vincristine was added to human multiple myeloma cell line ARH77 cultures for 24, 48, 72, and 96 h and 10-7 to 10-10 M concentrations. Sur-vivin expression was determined by quantitative RT-PCR. Statistical comparisons of ratios were made with two proportions z test.

Results:

We did not observe an increase in survivin expression after 24 or 96 h of treatment. Survivin expression significantly increased after 48 and 72 h (p<0,05)

Conclusion:

Expression of survivin in vincristine treated cells is dependent on time and concentration. Detailed studies are needed to evaluate changes in vincristine induced survivin overexpression in cells at the molecular level.