ÖZ
Amaç
Yaşlanma sırasında biriken reaktif oksijen türleri, böbrek ve testis dokularında oksidatif strese neden olarak kronik böbrek hastalığının ve erkek kısırlığının ilerlemesine katkıda bulunabilir. Kalori kısıtlaması (CR), oksidatif stresin modülasyonu da dahil olmak üzere çeşitli mekanizmalar aracılığıyla sağlıklı yaşlanmayı destekleyen en etkili farmakolojik olmayan yöntem olarak kabul edilir. Bu çalışma, kısa süreli CR’nin yaşlanma sırasında böbrek ve testis dokuları üzerindeki etkilerini araştırmayı amaçlamıştır.
Yöntemler
Yirmi sekiz sıçan, dört gruba ayrıldı: genç kontrol, genç CR, yaşlı kontrol ve yaşlı CR. Kontrol grupları al-libitum olarak beslenirken, CR grupları günlük kalori alımlarının yaklaşık üçte birini tüketti. On hafta sonra, kan parametreleri [kreatinin (Cre), kan üre azotu, ürik asit (UA), testosteron düzeyleri], oksidatif stres belirteçleri [malondialdehit (MDA) ve glutatyon (GSH)] ve histolojik değişiklikler analiz edildi.
Bulgular
Yaşlanma, vücut ve böbrek ağırlıklarında artış ve testis indeksinde azalma ile ilişkiliydi p < 0,005. CR, vücut ve böbrek ağırlıklarında azalma ve böbrek ve testis indekslerinde artış gösterdi (p < 0.005). Böbrek fonksiyon belirteçleri (Cre ve UA) yaşla birlikte artmış, ancak CR ile anlamlı olarak azalmıştı (p <0,005). Yaşlanma, hem böbrek hem de testis dokularında MDA düzeylerini artırdı ve GSH düzeylerini düşürdü. Yaşlanma, böbrek ve testis dokularında inflamatuar hücre infiltrasyonunu ve fibrozisi artırırken, CR yaşlı sıçanlarda fibrozis yüzdesini anlamlı şekilde azalttı.
Sonuç
Kısa süreli CR, oksidatif stresi azaltarak yaşlanma sırasında böbrek ve testisteki yapısal ve fonksiyonel bozuklukları hafifletebilir.
Anahtar Kelimeler:
Kalori kısıtlaması, yaşlanma, böbrek, testis, oksidatif stres, infertilite
Kaynaklar
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