ISSN: 2147-2092
Effects of Amantadine on Liver and Lung Tissue in Hepatic Ischemia Reperfusion Injury in Rats
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Original Investigation
VOLUME: 34 ISSUE: 1
P: 10-15
January 2023

Effects of Amantadine on Liver and Lung Tissue in Hepatic Ischemia Reperfusion Injury in Rats

GMJ 2023;34(1):10-15
DOI: 10.12996/gmj.2023.2
Fatih Sahin 1
Ayca Tas Tuna 2
Yusuf Unal 3
Mustafa Arslan 3
Hayrullah Yazar 4
Saban Cem Sezen 5
Sezen Irmak Gozukara 6
Onur Palabıyık 2
1. Sakarya University Training and Research Hospital, Department of Anesthesiology, Sakarya, Turkey
2. Sakarya University, Faculty of Medicine, Department of Anesthesiology, Sakarya, Turkey
3. Gazi University, Faculty of Medicine, Department of Anesthesiology, Ankara, Turkey
4. Sakarya University, Faculty of Medicine, Department of Biochemistry, Sakarya, Turkey
5. Kirikkale Uni versity, Faculty of Medicine, Department of Histology and Embryology, Kirikkale, Turkey
6. Sakarya University Training and Research Hospital, Department of Biochemistry, Sakarya, Turkey
No information available.
No information available
Received Date: 06.11.2021
Accepted Date: 09.05.2022
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ABSTRACT

Background:

N-Methyl D-Aspartate (NMDA) receptor blockers have been shown to have protective effects against ischemia/reperfusion (I/R) injury in various tissues. The aim of this study was to investigate the effects of amantadine on liver and lung tissue in hepatic I/R injury.

Materials and Methods:

Twenty-four rats divided into 4 groups: the Sham Group (S), the Amantadine Group (A), the I/R Group (I/R) and the I/R + Amantadine Group (I/R-A). In Group A and Group I/R-A, 45 mg/kg of amantadine was administered before surgery. In Group I/R and Group I/R-A, an atraumatic vascular clamp was applied to the structures in the left portal triad for 45 minutes and reperfusion period was 2 hours after declampage. Malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) enzyme levels were were studied in liver and lung tissues. Additionally tissues were examined histopathologically.

Results:

No significant difference was observed in tissue MDA, SOD, and CAT levels among four groups (p >0.05). Polymorphonuclear cell infiltration and the scores of hepatocyte degeneration, sinusoidal dilatation, pycnotic core, and necrosis cell were significantly higher in Group I/R than other groups (p<0.05). Regarding to the lung tissue, the neutrophil/lymphocyte infiltration score was significantly lower in Group S and A than in Group I/R (respectively; p= 0.007, 0.011), and it was significantly higher in Group I/R-A than in Group S (p = 0.014). The alveolar wall thickening score was significantly higher in Group I/R than the other groups (p <0.0001).

Conclusion:

Amantadine may have a protective effect against I/R damage, as it reduces histopathological changes caused by I/R damage.

Keywords:
Amantadine, Hepatic Ischemia-Reperfusion, Liver, Lung, Liver Surgery