ISSN: 2147-2092
Evaluating the Effect of Prebiotics in the Rehabilitation of Gut Microbiota After Antibiotic Therapy in Rats
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Original Investigation
VOLUME: 31 ISSUE: 1
P: 10-14
January 2020

Evaluating the Effect of Prebiotics in the Rehabilitation of Gut Microbiota After Antibiotic Therapy in Rats

GMJ 2020;31(1):10-14
DOI: 10.12996/gmj.2020.03
Rabia Omer 1
Sanaullah Iqbal 1
Bilal Sajid Mushtaq 2
Muhammad Bilal Hussain 3
Mustafa Nadhim Owaid 4,5
Farhan Saeed 3
Marwa Waheed 3
Mohammad Ali Shariati 6
Muhammad Usman Khan 7
Toheed Ahmad 1
1. Department of Food Science and Human Nutrition, University of Veterinary and Animal Sciences, Lahore, Pakistan
2. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, China
3. Institute of Home and Food Sciences, Government College University, Faisalabad, Pakistan
4. Department of Heet Education, General Directorate of Education in Anbar, Ministry of Education, Hit, Anbar, Iraq
5. Department of Ecology, College of Applied Sciences-Hit, University of Anbar, Hit, Anbar, Iraq
6. Laboratory of Biological Control and Antimicrobial Resistance, Orel State University named after I.S. Turgenev, Orel City, Russia
7. Department of Energy Systems Engineering, University of Agriculture, Faisalabad, Pakistan
No information available.
No information available
Received Date: 07.07.2018
Accepted Date: 02.03.2019
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ABSTRACT

The present study evaluated the effect of galacto-oligosaccharides (GOS) on gut microbiota after antibiotic treatment given two times a day. Four groups were made having six rats in each group. G1 was a control group fed on a basal diet. While, the remaining were treated in groups given antibiotic and GOS separately and also in combination as in G2. The dose of antibiotic and GOS was calculated by HED (Human Equivalent Dose) formula. Fecal samples were analyzed at the interval of five days for bacterial population especially Bifidobacterium spp., Lactobacillus spp. and Escherichia coli and total plate count was achieved using selective media. The results indicated that the growth of Bifidobacterium spp. and Lactobacillus spp. depended on GOS and antibiotic dose. The combination of GOS-Cephalexin is mostly of interest because due to the antibiotic. The results of Bifidobacterium spp. and Lactobacillus spp. were decreased while on GOS consumption, the growth of such species is increased. The results of G3 showed that the number of colonies of Bifidobacterium spp. and Lactobacillus spp. was significantly higher than G2 on the 5th day. Furthermore, the recovery rate was faster as compared to other groups. This research suggested that intake of GOS during antibiotic treatment significantly strengthen the microbiota by increasing the population of Bifidobacterium spp. and Lactobacillus spp. as well as reducing the number of E. coli that shows resistance to many antibiotics.