ABSTRACT

The goal of this research was to determine whether cerium oxide could protect against kidney tissue damage in rats given sevoflurane anesthesia.

After the approval of the Ethics Committee, study was conducted in Gazi University Animal Research Laboratory, Ankara, Turkey, in April 2019. 24 rats were divided into 4 groups: control group (C), cerium oxide group (CO), sevoflurane group (S), and cerium oxide-sevoflurane group (COS). 0.5 mg/kg doses of intraperitoneal cerium oxide was administered to the CO and COS groups 30 minutes before the procedure. Sevoflurane (2.3%) was administered to the S and COS groups for 3 hours. Histopathological and biochemical parameters were then analyzed.

Malondialdehyde (MDA) level and superoxide dismutase (SOD) activity were significantly higher in group S than in group C and CO. On the other hand, MDA level and SOD activity were significantly lower in group COS than in group S. Tubular dilatations markedly increased in group S and COS compared to group C (p=0.002, p=0.009, respectively). Tubular dilatation was also significantly higher in group S than in group CO. Tubular cell necrosis were significantly higher in all groups than in group C. Tubular cell necrosis was also significantly higher in group S compared to group CO. Bowman’s space dilatations were significantly higher in groups S and COS compared to the C group. Bowman’s space dilatation was also significantly higher in group S than in group CO. Tubular cell shedding was significantly higher in group S compared to group C and CO. Tubular cell necrosis and cell shedding were found to be significantly lower in group COS than in group S.

We conclude that pretreatment of rats with a single intraperitoneal dose of cerium oxide nanoparticles is safe to prevent kidney damage caused by sevoflurane anesthesia in rats.